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single hla class ii allele  (Addgene inc)


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    Addgene inc single hla class ii allele
    CD4 + T cell responses to SARS-CoV-2 antigens according to <t>HLA</t> class II loci and alleles. CD4 + T cells were stimulated with an allotype-matched aAPCs pulsed with a mixture of peptide pools of spike, nucleocapsid, and membrane protein. The responses by HLA-DR, -DQ, and -DP loci (A) , and HLA-DR alleles (B) , HLA-DQ alleles (C) , and HLA-DP alleles (D) in 42 donors were compared. Each dot presents the magnitude of response by a locus or an allele in an individual. Error bars indicate the mean ± standard error of the mean (SEM), and the number of donors with the allele are shown in parentheses. The stacked bar graph presents a proportion of the classified response in donors out of the donors with the allele. Statistical analysis was performed using a one-way ANOVA. *P < 0.05, ***P < 0.001. (E) Correlation of allele frequency with the proportion of positive response by HLA-DR, -DQ, and -DP alleles that are in at least three individuals. Statistical analysis was performed using Pearson’s correlation analysis. A line of best fit (solid lines) and the 95% confidence bands (dotted lines) were analyzed using linear regression analysis.
    Single Hla Class Ii Allele, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/single hla class ii allele/product/Addgene inc
    Average 93 stars, based on 11 article reviews
    single hla class ii allele - by Bioz Stars, 2026-06
    93/100 stars

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    1) Product Images from "Comprehensive Analysis of CD4 + T Cell Response Cross-Reactive to SARS-CoV-2 Antigens at the Single Allele Level of HLA Class II"

    Article Title: Comprehensive Analysis of CD4 + T Cell Response Cross-Reactive to SARS-CoV-2 Antigens at the Single Allele Level of HLA Class II

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2021.774491

    CD4 + T cell responses to SARS-CoV-2 antigens according to HLA class II loci and alleles. CD4 + T cells were stimulated with an allotype-matched aAPCs pulsed with a mixture of peptide pools of spike, nucleocapsid, and membrane protein. The responses by HLA-DR, -DQ, and -DP loci (A) , and HLA-DR alleles (B) , HLA-DQ alleles (C) , and HLA-DP alleles (D) in 42 donors were compared. Each dot presents the magnitude of response by a locus or an allele in an individual. Error bars indicate the mean ± standard error of the mean (SEM), and the number of donors with the allele are shown in parentheses. The stacked bar graph presents a proportion of the classified response in donors out of the donors with the allele. Statistical analysis was performed using a one-way ANOVA. *P < 0.05, ***P < 0.001. (E) Correlation of allele frequency with the proportion of positive response by HLA-DR, -DQ, and -DP alleles that are in at least three individuals. Statistical analysis was performed using Pearson’s correlation analysis. A line of best fit (solid lines) and the 95% confidence bands (dotted lines) were analyzed using linear regression analysis.
    Figure Legend Snippet: CD4 + T cell responses to SARS-CoV-2 antigens according to HLA class II loci and alleles. CD4 + T cells were stimulated with an allotype-matched aAPCs pulsed with a mixture of peptide pools of spike, nucleocapsid, and membrane protein. The responses by HLA-DR, -DQ, and -DP loci (A) , and HLA-DR alleles (B) , HLA-DQ alleles (C) , and HLA-DP alleles (D) in 42 donors were compared. Each dot presents the magnitude of response by a locus or an allele in an individual. Error bars indicate the mean ± standard error of the mean (SEM), and the number of donors with the allele are shown in parentheses. The stacked bar graph presents a proportion of the classified response in donors out of the donors with the allele. Statistical analysis was performed using a one-way ANOVA. *P < 0.05, ***P < 0.001. (E) Correlation of allele frequency with the proportion of positive response by HLA-DR, -DQ, and -DP alleles that are in at least three individuals. Statistical analysis was performed using Pearson’s correlation analysis. A line of best fit (solid lines) and the 95% confidence bands (dotted lines) were analyzed using linear regression analysis.

    Techniques Used:

    CD4 + T cell responses restricted by single HLA class II allotype within individuals. CD4 + T cells were stimulated with an allotype-matched aAPCs pulsed with a mixture of peptide pools of spike, nucleocapsid, and membrane protein. (A) CD4 + T cell responses (vertical) by two allotypes of HLA-DR, -DQ, and -DP loci (depth) in each individual (horizontal). The magnitude of response above 50 SFCs/5 × 10 5 are colored and the alleles corresponding to the response are shown in the same color. (B) A schematic diagram of the analysis of dominant response in individuals. (C) The order of highest response by an allotype within an individual is presented as the dominant response in an individual. Each dot represents a response by an allotype. Data are shown as mean ± standard deviation (SD) of the order-specific response. Statistical analysis was performed using one-way ANOVA. ****P < 0.0001. (D) The probability of intra-individual dominance presents (the number of donors with the corresponding order from the responses by the allotype)/(the number of donors with the allotype). The donors who did not respond by any allotype were excepted. The order and color of the allele follow (B) and (A) , respectively.
    Figure Legend Snippet: CD4 + T cell responses restricted by single HLA class II allotype within individuals. CD4 + T cells were stimulated with an allotype-matched aAPCs pulsed with a mixture of peptide pools of spike, nucleocapsid, and membrane protein. (A) CD4 + T cell responses (vertical) by two allotypes of HLA-DR, -DQ, and -DP loci (depth) in each individual (horizontal). The magnitude of response above 50 SFCs/5 × 10 5 are colored and the alleles corresponding to the response are shown in the same color. (B) A schematic diagram of the analysis of dominant response in individuals. (C) The order of highest response by an allotype within an individual is presented as the dominant response in an individual. Each dot represents a response by an allotype. Data are shown as mean ± standard deviation (SD) of the order-specific response. Statistical analysis was performed using one-way ANOVA. ****P < 0.0001. (D) The probability of intra-individual dominance presents (the number of donors with the corresponding order from the responses by the allotype)/(the number of donors with the allotype). The donors who did not respond by any allotype were excepted. The order and color of the allele follow (B) and (A) , respectively.

    Techniques Used: Standard Deviation

    CD4 + T cell responses by each HLA class II allotype to each SARS-CoV-2 antigen in each individual. (A, B) CD4 + T cell responses by an allotype were measured individually with spike (S), nucleocapsid (N), membrane (M), or CMV pp65, and with a mixture (MIX) of peptide pools of S, N, and M protein. High responses are highlighted at the blue end of the spectrum. (C) The order of immunogenicity indicates the order of highest response to S, N, or M among the responses by an allele that showed the strong response in an individual. Each dot represents a response by an allotype to a peptide pool of a protein. Data are shown as mean ± SD. Statistical analysis was performed using one-way ANOVA. *P < 0.05.
    Figure Legend Snippet: CD4 + T cell responses by each HLA class II allotype to each SARS-CoV-2 antigen in each individual. (A, B) CD4 + T cell responses by an allotype were measured individually with spike (S), nucleocapsid (N), membrane (M), or CMV pp65, and with a mixture (MIX) of peptide pools of S, N, and M protein. High responses are highlighted at the blue end of the spectrum. (C) The order of immunogenicity indicates the order of highest response to S, N, or M among the responses by an allele that showed the strong response in an individual. Each dot represents a response by an allotype to a peptide pool of a protein. Data are shown as mean ± SD. Statistical analysis was performed using one-way ANOVA. *P < 0.05.

    Techniques Used:



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    single hla class ii allele  (Addgene inc)
    93
    Addgene inc single hla class ii allele
    CD4 + T cell responses to SARS-CoV-2 antigens according to <t>HLA</t> class II loci and alleles. CD4 + T cells were stimulated with an allotype-matched aAPCs pulsed with a mixture of peptide pools of spike, nucleocapsid, and membrane protein. The responses by HLA-DR, -DQ, and -DP loci (A) , and HLA-DR alleles (B) , HLA-DQ alleles (C) , and HLA-DP alleles (D) in 42 donors were compared. Each dot presents the magnitude of response by a locus or an allele in an individual. Error bars indicate the mean ± standard error of the mean (SEM), and the number of donors with the allele are shown in parentheses. The stacked bar graph presents a proportion of the classified response in donors out of the donors with the allele. Statistical analysis was performed using a one-way ANOVA. *P < 0.05, ***P < 0.001. (E) Correlation of allele frequency with the proportion of positive response by HLA-DR, -DQ, and -DP alleles that are in at least three individuals. Statistical analysis was performed using Pearson’s correlation analysis. A line of best fit (solid lines) and the 95% confidence bands (dotted lines) were analyzed using linear regression analysis.
    Single Hla Class Ii Allele, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/single hla class ii allele/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    single hla class ii allele - by Bioz Stars, 2026-06
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    CD4 + T cell responses to SARS-CoV-2 antigens according to HLA class II loci and alleles. CD4 + T cells were stimulated with an allotype-matched aAPCs pulsed with a mixture of peptide pools of spike, nucleocapsid, and membrane protein. The responses by HLA-DR, -DQ, and -DP loci (A) , and HLA-DR alleles (B) , HLA-DQ alleles (C) , and HLA-DP alleles (D) in 42 donors were compared. Each dot presents the magnitude of response by a locus or an allele in an individual. Error bars indicate the mean ± standard error of the mean (SEM), and the number of donors with the allele are shown in parentheses. The stacked bar graph presents a proportion of the classified response in donors out of the donors with the allele. Statistical analysis was performed using a one-way ANOVA. *P < 0.05, ***P < 0.001. (E) Correlation of allele frequency with the proportion of positive response by HLA-DR, -DQ, and -DP alleles that are in at least three individuals. Statistical analysis was performed using Pearson’s correlation analysis. A line of best fit (solid lines) and the 95% confidence bands (dotted lines) were analyzed using linear regression analysis.

    Journal: Frontiers in Immunology

    Article Title: Comprehensive Analysis of CD4 + T Cell Response Cross-Reactive to SARS-CoV-2 Antigens at the Single Allele Level of HLA Class II

    doi: 10.3389/fimmu.2021.774491

    Figure Lengend Snippet: CD4 + T cell responses to SARS-CoV-2 antigens according to HLA class II loci and alleles. CD4 + T cells were stimulated with an allotype-matched aAPCs pulsed with a mixture of peptide pools of spike, nucleocapsid, and membrane protein. The responses by HLA-DR, -DQ, and -DP loci (A) , and HLA-DR alleles (B) , HLA-DQ alleles (C) , and HLA-DP alleles (D) in 42 donors were compared. Each dot presents the magnitude of response by a locus or an allele in an individual. Error bars indicate the mean ± standard error of the mean (SEM), and the number of donors with the allele are shown in parentheses. The stacked bar graph presents a proportion of the classified response in donors out of the donors with the allele. Statistical analysis was performed using a one-way ANOVA. *P < 0.05, ***P < 0.001. (E) Correlation of allele frequency with the proportion of positive response by HLA-DR, -DQ, and -DP alleles that are in at least three individuals. Statistical analysis was performed using Pearson’s correlation analysis. A line of best fit (solid lines) and the 95% confidence bands (dotted lines) were analyzed using linear regression analysis.

    Article Snippet: As previously described , 5 × 10 6 293TN producer cells (System Biosciences) were transfected (Lipofectamine 2000; Invitrogen) with 1.3 pmol psPAX2 (RRID: Addgene_12260), 0.72 pmol pMD.2G (RRID: Addgene_12259), and 1.64 pmol single HLA class II allele-encoding pCDH, and the supernatant was harvested after 2 days.

    Techniques:

    CD4 + T cell responses restricted by single HLA class II allotype within individuals. CD4 + T cells were stimulated with an allotype-matched aAPCs pulsed with a mixture of peptide pools of spike, nucleocapsid, and membrane protein. (A) CD4 + T cell responses (vertical) by two allotypes of HLA-DR, -DQ, and -DP loci (depth) in each individual (horizontal). The magnitude of response above 50 SFCs/5 × 10 5 are colored and the alleles corresponding to the response are shown in the same color. (B) A schematic diagram of the analysis of dominant response in individuals. (C) The order of highest response by an allotype within an individual is presented as the dominant response in an individual. Each dot represents a response by an allotype. Data are shown as mean ± standard deviation (SD) of the order-specific response. Statistical analysis was performed using one-way ANOVA. ****P < 0.0001. (D) The probability of intra-individual dominance presents (the number of donors with the corresponding order from the responses by the allotype)/(the number of donors with the allotype). The donors who did not respond by any allotype were excepted. The order and color of the allele follow (B) and (A) , respectively.

    Journal: Frontiers in Immunology

    Article Title: Comprehensive Analysis of CD4 + T Cell Response Cross-Reactive to SARS-CoV-2 Antigens at the Single Allele Level of HLA Class II

    doi: 10.3389/fimmu.2021.774491

    Figure Lengend Snippet: CD4 + T cell responses restricted by single HLA class II allotype within individuals. CD4 + T cells were stimulated with an allotype-matched aAPCs pulsed with a mixture of peptide pools of spike, nucleocapsid, and membrane protein. (A) CD4 + T cell responses (vertical) by two allotypes of HLA-DR, -DQ, and -DP loci (depth) in each individual (horizontal). The magnitude of response above 50 SFCs/5 × 10 5 are colored and the alleles corresponding to the response are shown in the same color. (B) A schematic diagram of the analysis of dominant response in individuals. (C) The order of highest response by an allotype within an individual is presented as the dominant response in an individual. Each dot represents a response by an allotype. Data are shown as mean ± standard deviation (SD) of the order-specific response. Statistical analysis was performed using one-way ANOVA. ****P < 0.0001. (D) The probability of intra-individual dominance presents (the number of donors with the corresponding order from the responses by the allotype)/(the number of donors with the allotype). The donors who did not respond by any allotype were excepted. The order and color of the allele follow (B) and (A) , respectively.

    Article Snippet: As previously described , 5 × 10 6 293TN producer cells (System Biosciences) were transfected (Lipofectamine 2000; Invitrogen) with 1.3 pmol psPAX2 (RRID: Addgene_12260), 0.72 pmol pMD.2G (RRID: Addgene_12259), and 1.64 pmol single HLA class II allele-encoding pCDH, and the supernatant was harvested after 2 days.

    Techniques: Standard Deviation

    CD4 + T cell responses by each HLA class II allotype to each SARS-CoV-2 antigen in each individual. (A, B) CD4 + T cell responses by an allotype were measured individually with spike (S), nucleocapsid (N), membrane (M), or CMV pp65, and with a mixture (MIX) of peptide pools of S, N, and M protein. High responses are highlighted at the blue end of the spectrum. (C) The order of immunogenicity indicates the order of highest response to S, N, or M among the responses by an allele that showed the strong response in an individual. Each dot represents a response by an allotype to a peptide pool of a protein. Data are shown as mean ± SD. Statistical analysis was performed using one-way ANOVA. *P < 0.05.

    Journal: Frontiers in Immunology

    Article Title: Comprehensive Analysis of CD4 + T Cell Response Cross-Reactive to SARS-CoV-2 Antigens at the Single Allele Level of HLA Class II

    doi: 10.3389/fimmu.2021.774491

    Figure Lengend Snippet: CD4 + T cell responses by each HLA class II allotype to each SARS-CoV-2 antigen in each individual. (A, B) CD4 + T cell responses by an allotype were measured individually with spike (S), nucleocapsid (N), membrane (M), or CMV pp65, and with a mixture (MIX) of peptide pools of S, N, and M protein. High responses are highlighted at the blue end of the spectrum. (C) The order of immunogenicity indicates the order of highest response to S, N, or M among the responses by an allele that showed the strong response in an individual. Each dot represents a response by an allotype to a peptide pool of a protein. Data are shown as mean ± SD. Statistical analysis was performed using one-way ANOVA. *P < 0.05.

    Article Snippet: As previously described , 5 × 10 6 293TN producer cells (System Biosciences) were transfected (Lipofectamine 2000; Invitrogen) with 1.3 pmol psPAX2 (RRID: Addgene_12260), 0.72 pmol pMD.2G (RRID: Addgene_12259), and 1.64 pmol single HLA class II allele-encoding pCDH, and the supernatant was harvested after 2 days.

    Techniques: